Human Interferon gamma ELISA Kit (IFNG)- 96 Tests

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Key features and details
Sensitivity: 15 pg/ml
Range: 20.6 pg/ml – 15000 pg/ml
Sample type: Cell culture supernatant, Plasma
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 19,488.00

Available on backorder

Detection method
Colorimetric
Sample type
Cell culture supernatant, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 15 pg/ml Range 20.6 pg/ml - 15000 pg/ml Recovery > 87 %

Sample specific recovery
Sample type Average % Range
Cell culture supernatant 94.53 84% – 104%
Plasma 86.82 81% – 102%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Interferon gamma (IFNG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Interferon gamma- in plasma, and cell culture supernatants. (Human Interferon gamma concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for Human Interferon gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Human Interferon gamma in normal serum with ab100537, only in serum spiked with Human Interferon gamma.

Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.

Notes
Optimization may be required with urine samples

Platform
Microplate

SKU: ab100537-1x 96 test Category: Tag:

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