Human Insulin ELISA Kit- 96 Tests

Product name
Human Insulin ELISA Kit
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 4 µlU/ml Range 4.69 µlU/ml - 300 µlU/ml Recovery 89 % Sample specific recovery Sample type Average % Range Cell culture supernatant 76.16 68% - 88% Serum 91.4 83% - 102% Plasma 99.03 73% - 128% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Predicted to work with: Mouse, Rat, Cow, Pig Product overview Abcam’s Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Insulin and Proinsulin in serum, plasma, cell culture supernatants. This assay employs an antibody specific for Human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This kit detects both insulin and proinsulin. Get higher sensitivity in only 90 minutes with Human Insulin ELISA Kit (ab200011) from our SimpleStep ELISA® range. Notes Optimization may be required with urine samples. Platform Microplate