Human TGF beta 1 ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 80 pg/ml
Range: 0.0823 ng/ml – 60 ng/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 19,488.00

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Detection method
Colorimetric
Precision
Intra-assay
Sample n Mean SD CV%
Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity < 80 pg/ml Range 0.082 ng/ml - 60 ng/ml Recovery 96 % Sample specific recovery Sample type Average % Range Cell culture supernatant 97.87 85% - 104% Serum 94.46 82% - 102% Plasma 95.78 93% - 103% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Human TGF beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100647) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Ab100647 was reformulated on 31st May 2018 with new capture and detector antibodies that allows to increase the sensitivity to human TGF beta 1. Please note that as a consequence of this change, the procedure and protocol have slightly changed and we encourage you to review the protocol steps before starting your experiments Notes Optimization may be required with urine samples. Platform Microplate

SKU: ab100647-1x 96 test Category: Tag:

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