Human TNF alpha ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 30 pg/ml Range 24.58 pg/ml - 6000 pg/ml Recovery 94 % Sample specific recovery Sample type Average % Range Cell culture supernatant 96.43 84% - 104% Serum 94.53 82% - 103% Plasma 91.92 80% - 102% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s TNF alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNF alpha in serum, plasma and cell culture supernatants. (Human TNF alpha concentration is pretty low in normal serum/plasma, it may not be detected in this assay). This assay employs an antibody specific for Human TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get higher sensitivity in only 90 minutes with Human TNF alpha ELISA Kit (ab181421) from our SimpleStep ELISA® range. Notes Optimization may be required with urine samples. Platform Microplate Properties