Human Prolactin ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 0.25 ng/ml
Range: 5 ng/ml – 100 ng/ml
Sample type: Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 18,480.00

Available on backorder

Sample type
Serum
Assay type
Sandwich (quantitative)
Sensitivity
0.25 ng/ml
Range
5 ng/ml – 100 ng/ml
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Prolactin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.

Microtiterstrip wells are precoated with inactivated specific Corynebacterium diphtheriae toxin (toxoid) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured C. diphtheriae toxin-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of C. diphtheriae toxin-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

Get results in 90 minutes with Human Prolactin ELISA Kit (ab189570) from our SimpleStep ELISA® range.

Platform
Microplate

SKU: ab108679-1x 96 test Category: Tag:

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