Human Insulin like Growth Factor 1 ELISA Kit (IGF1)- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 0.4 ng/ml
Range: 0.4 ng/ml – 24 ng/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,152.00
Available on backorder
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
> 0.4 ng/ml
Range
0.4 ng/ml – 24 ng/ml
Recovery
= 98 %
Assay time
5h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Insulin like Growth Factor 1 (IGF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin like Growth Factor 1 concentrations in Human cell culture supernatants, serum and plasma.
An Insulin like Growth Factor 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Insulin like Growth Factor 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Insulin like Growth Factor 1 captured in plate.
Get results in 90 minutes with Human IGF1 ELISA Kit (ab211651) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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