17 beta Estradiol ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 8.68 pg/ml
Range: 20 pg/ml – 2000 pg/ml
Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
Detection method: Colorimetric
Assay type: Competitive
Reacts with: Mouse, Rat, Human

EGP 19,152.00

Available on backorder

Sample type
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay type
Competitive
Sensitivity
8.68 pg/ml
Range
20 pg/ml – 2000 pg/ml
Recovery
101.09 %

Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Mammals
Product overview
Abcam’s 17 beta Estradiol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 beta Estradiol in serum and plasma (citrate).

A 96-well plate has been precoated with anti-Estradiol IgG. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.

Platform
Microplate

SKU: ab108667-1x 96 test Category: Tag:

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