Androstenedione ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 0.01 ng/ml
Range: 0.1 ng/ml – 10 ng/ml
Sample type: Cit plasma, Hep Plasma, Serum
Detection method: Colorimetric
Assay type: Competitive

EGP 19,152.00

Available on backorder

Sample type
Serum, Hep Plasma, Cit plasma
Assay type
Competitive
Sensitivity
= 0.01 ng/ml
Range
0.1 ng/ml – 10 ng/ml
Recovery
100.91 %

Assay duration
Multiple steps standard assay
Product overview
Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in serum and plasma.

A 96-well plate has been precoated with anti-Androstenedione IgG. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.

Platform
Microplate

SKU: ab108672-1x 96 test Category: Tag:

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