Sample type
Cell culture supernatant, Urine
Assay type
Sandwich (quantitative)
Sensitivity
= 12 ng/ml
Range
12.5 ng/ml – 800 ng/ml
Recovery
97 %
Assay time
4h 0m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Dog
Product overview
Abcam’s Fibrinogen Canine in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of fibrinogen levels in urine and cell culture media.
A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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