Corticosterone ELISA kit- 96 Tests

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Key features and details
Sensitivity: 0.3 ng/ml
Range: 2 ng/ml – 22 ng/ml
Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
Detection method: Colorimetric
Assay type: Competitive
Reacts with: Mouse, Rat, Human, Baboon

EGP 19,152.00

Available on backorder

Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
Assay type
Competitive
Sensitivity
= 0.3 ng/ml
Range
2 ng/ml – 22 ng/ml
Recovery
= 101 %

Assay time
3h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse, Rat, Human, Baboon
Product overview
Corticosterone ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, saliva and cell culture supernatant.

A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate.

Please note the concentrations of the kit components are lot-specific and the end user should always refer to the vial label

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate

SKU: ab108821-1x 96 test Category: Tag:

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