Cortisol ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 2.95 ng/ml
Range: 10 ng/ml – 500 ng/ml
Sample type: Urine
Detection method: Colorimetric
Assay type: Competitive
Reacts with: Human

EGP 19,152.00

Available on backorder

Sample type
Urine
Assay type
Competitive
Sensitivity
2.95 ng/ml
Range
10 ng/ml – 500 ng/ml
Recovery
107.48 %

Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in urine.

A 96-well plate has been precoated with anti-Cortisol. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

Platform
Microplate

SKU: ab108674-1x 96 test Category: Tag:

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