Sample type
Serum, Hep Plasma, Cit plasma
Assay type
Competitive
Sensitivity
0.22 ng/ml
Range
2 ng/ml – 200 ng/ml
Recovery
94.88 %
Assay duration
Multiple steps standard assay
Product overview
Abcam’s Estriol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estriol in serum and plasma.
A 96-well plate has been precoated with anti-Estriol. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.
Platform
Microplate
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