Gardnerella vaginalis/Lactobac. species Real-TM Quant with silica DNA extraction kit 100 tests

Bacterial vaginosis (BV) is considered to be the most common cause of vaginal inflammation among both pregnant and non-pregnant women and prevalences between 4.9% and 36.0% have been reported from European and American studies. It previously was called nonspecific vaginitis or Gardnerella-associated vaginitis.
The adult human vagina is a complex ecosystem containing an abundance of microorganisms. In women of childbearing age this system is dominated by Lactobacillus spp., a genus of gram-positive, non-motile rod-like bacteria, a defining characteristic of which is the ability to grow in acid media and tolerate acid conditions (pH <4.5); lactobacilli also ferment carbohydrates to produce lactic acid and produce H2O2 which provides a natural defense against Gardnerella vaginalis. In bacterial vaginosis (BV) the balance of flora is changed with reduced numbers of lactobacilli (normal concentration 10^6-10^10 CFU/ml) and an increase in numbers of other facultative and anaerobic species such as anaerobic cocci Prevotella spp., Gardnerella vaginalis, and Mobiluncus spp. (normal concentration < 10^3-10^5 CFU/ml). G. vaginalis is virtually always present at high concentrations in women who have BV but is also detected frequently in normal women and in some cases the concentration of Gardnerella vaginalis can reach 10^7-10^8 CFU/ml also in absence of BV, so the most important maker of BV is the ratio of logarithm concentration Lactobacillus spp and G. vaginalis. The clinical significance of studying vaginal flora is that it helps determine the quantity of microorganisms and assess the ratio between the different groups of conditionally pathogenic microorganisms and the normal flora. The total quantity of bacteria serves as an indicator of infection level in the vaginal enviroment: under normal conditions it can vary between 10^6 and 10^9 (6-9 Log). The ratio between lactobacilli and the total bacterial quantity can be used as an indicator of the balance between the normal and conditionally pathogenic flora: the normal proportion of lactobacilli should be 95 to 100% of the total bacterial quantity. Bacterial Vaginosis Real-TM Quant kit is for the quantitative detection of Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp. and total bacteriae quantity in the vaginal biotope. The logarithmic ratio between Lactobacillus spp. and the total quantity of bacteriae and also between Lactobacillus spp. and facultative pathogen microorganisms (Gardnerella vaginalis and Atopobium vaginae) allows to detect with high precision bacterial vaginosis disease related to normal vaginal microflora suppression (Lactobacillus spp.) and substitution with facultative pathogen microorganisms (Gardnerella vaginalis and Atopobium vaginae). The use of Bacterial Vaginosis Real-TM Quant kit allows to monitor vaginal environment condition and to check the therapy efficiency.