Human Adiponectin ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 0.7 ng/ml
Range: 0.781 ng/ml – 50 ng/ml
Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 19,152.00

Available on backorder

Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
= 0.7 ng/ml
Range
0.781 ng/ml – 50 ng/ml
Recovery
99 %

Assay time
3h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Adiponectin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Adiponectin levels in urine, plasma, serum, and milk, saliva, and cell culture supernatants. (UniprotID: Q15848)

An Adiponectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate

SKU: ab108786-1x 96 test Category: Tag:

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