Sample type
Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
Assay type
Sandwich (quantitative)
Sensitivity
= 0.94 ng/ml
Range
12.5 ng/ml – 100 ng/ml
Recovery
97 %
Assay time
3h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Human Albumin ELISA kit (ab108788) is a sandwich ELISA kit designed for the quantitative measurement of albumin levels in urine, saliva, milk, cerebrospinal fluids and cell culture samples.
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.
Get results in 90 minutes with Human Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.
Please note: For both the 10X Diluent N Concentrate and 20X Wash Buffer Concentrate, please ensure to mix well by inversion before use to avoid precipitation.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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