Human Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AGP)- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 2.3 ng/ml
Range: 12.5 ng/ml – 100 ng/ml
Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,152.00
Available on backorder
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
Assay type
Sandwich (quantitative)
Sensitivity
= 2.3 ng/ml
Range
12.5 ng/ml – 100 ng/ml
Recovery
= 96 %
Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Alpha 1 Acid Glycoprotein / AGP Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of AGP levels in cell culture supernatant, saliva, milk, and urine.
An Alpha 1 Acid Glycoprotein / AGP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Alpha 1 Acid Glycoprotein / AGP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Alpha 1 Acid Glycoprotein / AGP captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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