Human alpha 1 Microglobulin ELISA Kit- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 0.16 ng/ml
Range: 0.313 ng/ml – 20 ng/ml
Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,152.00
Available on backorder
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
Assay type
Sandwich (quantitative)
Sensitivity
= 0.16 ng/ml
Range
0.313 ng/ml – 20 ng/ml
Recovery
96 %
Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s alpha 1 Microglobulin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 Microglobulin concentrations in Human cell culture supernatants, serum, plasma, urine, saliva, CSF and milk.
An alpha 1 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 Microglobulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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