Human alpha 2 Macroglobulin ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 0.95 ng/ml
Range: 1 ng/ml – 25 ng/ml
Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 19,152.00

Available on backorder

Sample type
Cell culture supernatant, Saliva, Milk, Cerebral Spinal Fluid
Assay type
Sandwich (quantitative)
Sensitivity
= 0.95 ng/ml
Range
1 ng/ml – 25 ng/ml
Recovery
97 %

Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s alpha 2 Macroglobulin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in saliva, milk, cerebrospinal fluid and cell culture supernatants.

An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 2 Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 2 Macroglobulin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate

SKU: ab108883-1x 96 test Category: Tag:

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