Sample type
Serum, Hep Plasma, Cit plasma, Cerebral Spinal Fluid
Assay type
Indirect
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s anti-Borrelia burgdorferi IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF (cerebrospinal fluid).
A 96-well plate has been precoated with recombinant Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgM sample captured in plate.
Platform
Microplate
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