Sample type
Serum, Hep Plasma, Cit plasma
Assay type
Indirect
Sensitivity
3 U/ml
Range
3 U/ml – 150 U/ml
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s anti-Helicobacter pylori IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Helicobacter pylori in Human serum and plasma.
A 96-well plate has been precoated with Helicobacter pylori antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Helicobacter pylori-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Helicobacter pylori IgG sample captured in plate.
Platform
Microplate
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