Human CXCL1 ELISA Kit (GRO alpha)- 96 Tests
Available on backorder
Expiry Date
Key features and details
Sensitivity: 2 pg/ml
Range: 2 pg/ml – 1000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,488.00
Available on backorder
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
2 pg/ml
Range
2 pg/ml – 1000 pg/ml
Recovery
> 99 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 112.2 101% – 122%
Serum 130.4 119% – 145%
Plasma 113.5 85% – 140%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s CXCL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit (GRO alpha) is an in vitro enzyme designed for the quantitative measurement of Human GRO alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CXCL1 (GRO alpha) coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human CXCL1 ELISA Kit (GRO alpha) (ab190805) from our SimpleStep ELISA® range.
Notes
Optimization may be required with urine samples
Platform
Microplate
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