Human CXCL7 ELISA Kit- 96 Tests
Available on backorder
Expiry Date
Key features and details
Sensitivity: 8.5 pg/ml
Range: 4.1 pg/ml – 1000 pg/ml
Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,488.00
Available on backorder
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma, Cell Lysate
Assay type
Sandwich (quantitative)
Sensitivity
< 8.5 pg/ml
Range
4.1 pg/ml - 1000 pg/ml
Recovery
93 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 95.28 84% - 103%
Serum 92.76 82% - 102%
Plasma 93.45 83% - 103%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s CXCL7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL7 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Notes
Optimization may be required with urine samples.
Platform
Microplate
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