Sample type
Cell culture supernatant, Saliva, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
= 6 ng/ml
Range
6.25 ng/ml – 200 ng/ml
Recovery
97 %
Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Factor VII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor VII concentrations in Human cell culture supernatants, serum and plasma.
A Factor VII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor VII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor VII captured in plate. This assay recognizes FVII and FVIIa.
Get higher sensitivity in only 90 minutes with Human Factor VII ELISA Kit (ab190810) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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