Sample type
Serum, Hep Plasma, Cit plasma
Assay type
Sandwich (quantitative)
Sensitivity
0.53 ng/ml
Recovery
98.66 %
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Human Ferritin ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Ferritin (FTL) in serum and plasma.
A 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.
Get results in 90 minutes with Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.
Platform
Microplate
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