Human Fibronectin ELISA Kit- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 4 ng/ml
Range: 0.004 µg/ml – 1 µg/ml
Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva, Urine
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,152.00
Available on backorder
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
Assay type
Sandwich (quantitative)
Sensitivity
= 4 ng/ml
Range
0.004 µg/ml – 1 µg/ml
Recovery
97.5 %
Assay time
3h 30m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Fibronectin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin levels in urine, saliva, milk, cerebrospinal fluid and cell culture supernatants.
A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.
Get results in 90 minutes with Human Fibronectin ELISA Kit (ab181419) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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