Human Free Estriol ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 0.05 ng/ml
Range: 0.1 ng/ml – 12 ng/ml
Sample type: Cit plasma, Hep Plasma, Serum
Detection method: Colorimetric
Assay type: Competitive
Reacts with: Human

EGP 17,976.00

Available on backorder

Detection method
Colorimetric
Sample type
Serum, Hep Plasma, Cit plasma
Assay type
Competitive
Sensitivity
= 0.05 ng/ml
Range
0.1 ng/ml – 12 ng/ml
Recovery
101.6 %

Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Free Estriol competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Free Estriol in serum and plasma.

A 96-well plate has been precoated with anti-Estriol IgG. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.

Platform
Microplate

SKU: ab108671-1x 96 test Category: Tag:

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