Human G-CSF ELISA Kit- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 1 pg/ml
Range: 0.69 pg/ml – 500 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,488.00
Available on backorder
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 1 pg/ml
Range
0.69 pg/ml - 500 pg/ml
Recovery
> 75 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 76.56 68% – 90%
Serum 83.45 72% – 94%
Plasma 74.76 67% – 87%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s G-CSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human G-CSF in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human G-CSF ELISA Kit (CSF3) (ab188390) from our SimpleStep ELISA® range.
Notes
Optimization may be required with urine samples
Platform
Microplate
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