Human GC Globulin ELISA Kit (Vitamin D Binding Protein)- 96 Tests

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Key features and details
Sensitivity: 0.075 µg/ml
Range: 0.098 µg/ml – 100 µg/ml
Sample type: Plasma, Serum
Detection method: Colorimetric
Assay type: Competitive
Reacts with: Human

EGP 19,152.00

Available on backorder

Sample type
Serum, Plasma
Assay type
Competitive
Sensitivity
> 0.075 µg/ml
Range
0.098 µg/ml – 100 µg/ml
Recovery
97 %

Assay time
3h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Human GC Globulin (Vitamin D Binding Protein) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of GC Globulin in plasma, serum, saliva, milk and cell-culture supernatants.

A GC Globulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated GC Globulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of GC Globulin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate

SKU: ab108853-1x 96 test Category: Tag:

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