Detection method
Colorimetric
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Sandwich (quantitative)
Sensitivity
< 8 pg/ml
Range
8.23 pg/ml - 6000 pg/ml
Recovery
95 %
Sample specific recovery
Sample type Average % Range
Cell culture extracts 95 81% - 104%
Tissue Extracts 95.5 80% - 103%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Human HGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HGF in cell lysates and tissue lysates.
This assay employs an antibody specific for Human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Microplate
Elisa Kits
Human HGF ELISA Kit- 96 Tests
Availability:
Available on backorder
Expiry Date
Key features and details
Sensitivity: 8 pg/ml
Range: 8.23 pg/ml – 6000 pg/ml
Sample type: Cell culture extracts, Tissue Extracts
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,488.00
Available on backorder
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