Human IgG ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 20 pg/ml
Range: 0.02 ng/ml – 15 ng/ml
Sample type: Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 19,488.00

Available on backorder

Detection method
Colorimetric
Sample type
Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 20 pg/ml Range 0.02 ng/ml - 15 ng/ml Recovery 117 % Sample specific recovery Sample type Average % Range Serum 116.1 105% - 123% Plasma 118.1 99% - 126% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Human IgG ELISA kit is designed for the quantitative measurement of Human IgG in serum and plasma. (UniprotID: Q60590) Notes The Human immune system consists of two functional components classified as the innate system (the physical, biochemical and cellular barriers), and the adaptive immune system (including lymphocytes and immunoglobulins). Immunoglobulins are the key elements of the humoral immune response in vertebrate against parasitic invasion. The polypeptide chains of immunoglobulins composed of two identical heavy (H) chains and two identical light (L) chains linked together by inter-chain disulfide bonds. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membranes. Based upon the variation of the constant region of the heavy chain, nine immunoglobulin heavy chain isotypes are found in humans: IgA (with subclasses IgA1 and IgA2), IgD, IgE, IgM, and IgG (with subclasses IgG1, IgG2, IgG3, and IgG4). IgG is the predominant immunoglobulin in the serum, which accounts for 75% of the total serum antibody of healthy individuals. IgG has a molecular weight of about 150 kDa. Four distinct subgroups of human IgG (IgG1, IgG2, IgG3, and IgG4) were first demonstrated in the 1960’s by using polyclonal antisera prepared in animals immunized with human myeloma proteins. The capture antibody of the kit ab100547 detects the Fc portion of the heavy chain of all subclasses of human IgG and the detection antibody detects the F(ab’)2 fragment of IgG. Platform Microplate

SKU: ab100547-1x 96 test Category: Tag:

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