Human IL-10 ELISA Kit- 96 Tests

Detection method
Colorimetric
Precision
Intra-assay
Sample n Mean SD CV%
Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity < 1 pg/ml Range 2.34 pg/ml - 150 pg/ml Recovery 100 % Sample specific recovery Sample type Average % Range Cell culture supernatant 98.37 87% - 110% Serum 101.94 87% - 108% Plasma 102.39 93% - 109% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s IL-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA® range. Notes Optimization may be required with urine samples Platform Microplate