Human IL-10RB ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 5 pg/ml
Range: 4.12 pg/ml – 3000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 20,160.00

Available on backorder

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 5 pg/ml Range 4.12 pg/ml - 3000 pg/ml Recovery > 100 %

Sample specific recovery
Sample type Average % Range
Cell culture supernatant 129.1 120% – 140%
Serum 121.9 110% – 129%
Plasma 104.5 92% – 112%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s IL-10RB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IL-10RB in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human IL-10RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Notes
Optimization may be required with urine samples

Platform
Microplate

SKU: ab100550-1x 96 test Category: Tag:

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