Human Lysozyme ELISA Kit (LZM)- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 0.15 ng/ml
Range: 0.156 ng/ml – 5 ng/ml
Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Other biological fluids, Plasma, Saliva, Serum, Urine
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,152.00
Available on backorder
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Other biological fluids, Cerebral Spinal Fluid
Assay type
Sandwich (quantitative)
Sensitivity
= 0.15 ng/ml
Range
0.156 ng/ml – 5 ng/ml
Recovery
= 97.5 %
Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Human Lysozyme (LZM) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human Lysozyme in plasma, serum, urine, saliva, CSF and cell culture supernatants.
A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lysozyme specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lysozyme captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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