Human Myoglobin ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 5 ng/ml
Range: 5 ng/ml – 1000 ng/ml
Sample type: Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 19,488.00

Available on backorder

Detection method
Colorimetric
Sample type
Serum
Assay type
Sandwich (quantitative)
Sensitivity
= 5 ng/ml
Range
5 ng/ml – 1000 ng/ml
Recovery
102.8 %

Sample specific recovery
Sample type Average % Range
Serum 102.8 39ng/ml – 780ng/ml
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Myoglobin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myoglobin in Human serum.

A 96-well plate has been precoated with anti-Myoglobin antibodies. Samples and standards and are added to the wells, where Myoglobin in the sample and standards binds to the precoated antibody. Added anti-Myoglobin HRP conjugate binds to this antibody-Myoglobin complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Myoglobin in the sample and the intensity is measured at 450 nm.

Platform
Microplate

SKU: ab108652-1x 96 test Category: Tag:

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