Human Osteopontin ELISA Kit- 96 Tests

Detection method
Colorimetric
Sample type
Cell culture supernatant, Milk, Urine, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 50 pg/ml Range 74.07 pg/ml - 18000 pg/ml Recovery 95 % Sample specific recovery Sample type Average % Range Cell culture supernatant 103.5 95% - 118% Serum 89.92 88% - 108% Plasma 92.41 90% - 109% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s Osteopontin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Osteopontin in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Get higher sensitivity in only 90 minutes with Human Osteopontin ELISA Kit (ab192143) from our SimpleStep ELISA® range. Notes Optimization may be required with urine samples Platform Microplate