Human PAI1 + tPA ELISA Kit- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 0.06 ng/ml
Range: 0.063 ng/ml – 4 ng/ml
Sample type: Cell culture supernatant, Plasma, Serum, Tissue
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,152.00
Available on backorder
Sample type
Cell culture supernatant, Serum, Plasma, Tissue
Assay type
Sandwich (quantitative)
Sensitivity
= 0.06 ng/ml
Range
0.063 ng/ml – 4 ng/ml
Recovery
98 %
Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s PAI1 + tPA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 + tPA concentrations in plasma, serum, tissue extracts and cell culture supernatants.
A PAI1 + tPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 + tPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 + tPA captured in plate.
Get results in 90 minutes with Human PAI1+tPA ELISA Kit (ab192151) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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