Sample type
Plasma, Platelets, Cell Lysate
Assay type
Sandwich (quantitative)
Sensitivity
= 0.5 ng/ml
Range
1.25 ng/ml – 40 ng/ml
Recovery
101 %
Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Platelet Glycoprotein IIb + IIIa Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Platelet Glycoprotein IIb+IIIa concentrations in Human platelets, platelet-rich plasma and cell culture lysate.
A Platelet Glycoprotein llb + llla specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Platelet Glycoprotein llb+llla specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Platelet Glycoprotein llb + llla captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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