Human Procalcitonin ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 20 pg/ml
Range: 27.43 pg/ml – 20000 pg/ml
Sample type: Cell culture supernatant, Plasma
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 19,488.00

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Product name
Human Procalcitonin ELISA Kit
Detection method
Colorimetric
Sample type
Cell culture supernatant, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 20 pg/ml Range 27.43 pg/ml - 20000 pg/ml Recovery 83 % Sample specific recovery Sample type Average % Range Cell culture supernatant 100.5 80% - 111% Plasma 72.35 65% - 88% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Abcam’s Procalcitonin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Procalcitonin in plasma and cell culture supernatants. (Human Procalcitonin concentration is pretty low in normal plasma, it may not be detected in this assay). This assay employs an antibody specific for Human Procalcitonin coated on a 96-well plate. Standards and samples are pipetted into the wells and Procalcitonin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Procalcitonin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Procalcitonin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. We have not been able to detect endogenous Human Procalcitonin in normal serum with ab100630, only in serum spiked with Human Procalcitonin. Notes Optimization may be required with urine samples. Platform Microplate

SKU: ab100630-1x 96 test Category: Tag:

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