Detection method
Colorimetric
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay type
Competitive
Sensitivity
0.05 ng/ml
Range
0.2 ng/ml – 40 ng/ml
Recovery
106 %
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Predicted to work with: Mammals
Product overview
Abcam’s Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Progesterone in serum and plasma.
A 96-well plate has been precoated with anti-Progesterone. Samples and the Progesterone-HRP conjugate are added to the wells, where Progesterone in the sample competes with the added Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Progesterone in the sample and the intensity is measured at 450 nm.
Platform
Microplate
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