Human SAA ELISA Kit (SAA1)- 96 Tests

Product name
Human SAA ELISA Kit (SAA1)
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 0.5 ng/ml Range 0.41 ng/ml - 300 ng/ml Recovery 100 % Sample specific recovery Sample type Average % Range Cell culture supernatant 93.75 86% - 102% Serum 114.8 105% - 124% Plasma 112.1 83% - 137% Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Product overview Human SAA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SAA in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human Serum Amyloid A coated on a 96-well plate. Standards and samples are pipetted into the wells and Serum Amyloid A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Serum Amyloid A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Serum Amyloid A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Notes Optimization may be required with urine samples. ab100635 cross reacts with SAA2. Platform Microplate