Human Thrombin-Antithrombin Complex ELISA Kit (TAT)- 96 Tests

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Key features and details
Sensitivity: 0.84 ng/ml
Range: 0.84 ng/ml – 120 ng/ml
Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Milk, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 19,152.00

Available on backorder

Sample type
Cell culture supernatant, Milk, Serum, EDTA Plasma, Cit plasma
Assay type
Sandwich (quantitative)
Sensitivity
= 0.84 ng/ml
Range
0.84 ng/ml – 120 ng/ml
Recovery
95 %

Assay time
4h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Thrombin-Antithrombin Complex (TAT) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin-Antithrombin Complex concentrations in plasma, milk and cell culture supernatants.

A Thrombin-Antithrombin Complex specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin-Antithrombin Complex specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin-Antithrombin Complex captured in plate.

Note: It is not recommended to perform spike recovery in plasma samples due to large amount of endogenous Anti-thrombin III (mg/ml range) which will saturate the capture antibody and interfere with the binding of the TAT standard

The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.

Platform
Microplate

SKU: ab108907-1x 96 test Category: Tag:

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