Human Thyroxine ELISA Kit (fT4)- 96 Tests

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Key features and details
Sensitivity: 0.5 pg/ml
Sample type: Cit plasma, Hep Plasma, Serum
Detection method: Colorimetric
Assay type: Competitive
Reacts with: Human

EGP 18,480.00

Available on backorder

Detection method
Colorimetric
Sample type
Serum, Hep Plasma, Cit plasma
Assay type
Competitive
Sensitivity
0.5 pg/ml
Recovery
97.8 %

Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Thyroxine (fT4) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of free Thyroxine in Human serum and plasma.

A 96-well plate has been precoated with anti-Thyroxine antibodies. Samples and the Thyroxine-HRP conjugate are added to the wells, where Thyroxine in the sample competes with the added
Thyroxine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Thyroxine in the sample and the intensity is measured at 450 nm.

Platform
Microplate

SKU: ab108686-1x 96 test Category: Tag:

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