Detection method
Colorimetric
Sample type
Serum
Assay type
Competitive
Sensitivity
= 0.4 µg/dl
Range
0.4 µg/dl – 25 µg/dl
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
ab108661, Thyroxine (T4) Human ELISA Kit is intended for the quantitative determination of Total Thyroxine (T4) concentration in Human serum.
L-Thyroxine (T4) is a hormone that is synthesized and stored in the thyroid gland. Proteolytic cleavage of follicular thyroglobulin releases T4 into the bloodstream. Greater than 99% of Thyroxine (T4) is reversibly bound to three plasma proteins in blood – thyroxine binding globulin (TBG) binds 70%, thyroxine binding pre-albumin (TBPA) binds 20%, and albumin binds 10%. Approximately 0.03% of T4 is in the free, unbound state in blood at any one time.
Platform
Microplate
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