Sample type
Cell culture supernatant, Milk, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
> 0.12 ng/ml
Range
0.313 ng/ml – 20 ng/ml
Recovery
96.5 %
Assay time
5h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Tissue Factor Pathway Inhibitor (TFPI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor Pathway Inhibitor concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture samples.
A Tissue Factor Pathway Inhibitor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor Pathway Inhibitor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor Pathway Inhibitor captured in plate.
Get results in 90 minutes with Human Tissue Factor Pathway Inhibitor ELISA Kit (ab188392) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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