Human Tissue Type Plasminogen Activator ELISA Kit (TPA)- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 0.03 ng/ml
Range: 0.031 ng/ml – 2 ng/ml
Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Tissue Extracts, Urine
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,152.00
Available on backorder
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue Extracts
Assay type
Sandwich (quantitative)
Sensitivity
= 0.03 ng/ml
Range
0.031 ng/ml – 2 ng/ml
Recovery
98 %
Assay time
4h 0m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Tissue Type Plasminogen Activator (TPA) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Type Plasminogen Activator in plasma, serum, urine, saliva, milk, cell culture supernatants and tissue extracts.
A TPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TPA captured in plate.
Get results in 90 minutes with Human Tissue Plasminogen Activator ELISA Kit (ab190812) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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