Human TREM1 ELISA Kit- 96 Tests
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Expiry Date
Key features and details
Sensitivity: 70 pg/ml
Range: 0.069 ng/ml – 50 ng/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,488.00
Available on backorder
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 70 pg/ml
Range
0.069 ng/ml - 50 ng/ml
Recovery
> 100 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 124.4 106% – 134%
Serum 125.5 91% – 145%
Plasma 116 94% – 115%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s TREM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TREM1 in serum, plasma and cell culture supernatants. (Human TREM1 concentration is pretty low in normal serum, it may not be detected in this assay).
This assay employs an antibody specific for Human TREM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TREM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TREM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TREM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human soluble TREM1 ELISA Kit (ab185987) from our SimpleStep ELISA® range.
Notes
Optimization may be required with urine samples.
Platform
Microplate
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