Human Triiodothyronine ELISA Kit (free + total T3)- 96 Tests

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Key features and details
Sensitivity: 0.2 ng/ml
Range: 0.2 ng/ml – 10 ng/ml
Sample type: Serum
Detection method: Colorimetric
Assay type: Competitive
Reacts with: Human

EGP 19,488.00

Available on backorder

Detection method
Colorimetric
Sample type
Serum
Assay type
Competitive
Sensitivity
= 0.2 ng/ml
Range
0.2 ng/ml – 10 ng/ml
Recovery
106 %

Sample specific recovery
Sample type Average % Range
Serum 106 0.68ng/ml – 5.81ng/ml
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Triiodothyronine (T3) in Human serum.

A 96-well plate has been pre-coated with goat anti-mouse IgG. Samples, standards and the mouse anti-Triiodothyronine Reagent and Triiodothyronine-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the added Triiodothyronine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.

This kit detects total and free T3. To detect only free T3 please use ab108663.

Platform
Microplate

SKU: ab108664-1x 96 test Category: Tag:

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