Human VEGF ELISA Kit- 96 Tests

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Key features and details
Sensitivity: 10 pg/ml
Range: 8.23 pg/ml – 6000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

EGP 19,488.00

Available on backorder

Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 10 pg/ml Range 8.23 pg/ml - 6000 pg/ml Recovery > 100 %

Sample specific recovery
Sample type Average % Range
Cell culture supernatant 103.5 92% – 113%
Serum 104.4 92% – 115%
Plasma 105.7 93% – 114%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Notes
Optimization may be required with urine samples.

Platform
Microplate

SKU: ab100662-1x 96 test Category: Tag:

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