Human Von Willebrand Factor ELISA Kit (VWF)- 96 Tests
Available on backorder
Expiry Date
Key features and details
Sensitivity: 0.38 mIU/ml
Range: 0.625 mIU/ml – 40 mIU/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human
EGP 19,152.00
Available on backorder
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
= 0.38 mIU/ml
Range
0.625 mIU/ml – 40 mIU/ml
Recovery
98 %
Assay time
5h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Human Von Willebrand Factor (VWF) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Von Willebrand Factor in plasma, serum, and cell culture supernatants.
A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.
Get higher sensitivity in only 90 minutes with Human Von Willebrand Factor ELISA Kit (ab189571) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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