Sample type
Cell culture supernatant, Urine, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
> 0.28 ng/ml
Range
0.781 ng/ml – 200 ng/ml
Recovery
97 %
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Mouse
Product overview
Mouse Albumin ELISA kit (ab108792) is a sandwich ELISA kit designed for the quantitative measurement of mouse Albumin levels in urine and cell culture supernatants.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
The entire kit may be stored at -20°C for long term storage before reconstitution – Avoid repeated freeze-thaw cycles.
Platform
Microplate
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